Цель

Изучение генетической структуры овец волгоградской и эдильбаевской пород по генам GDF9, GH.

Материалы и методы

Исследования проводили в стадах овец ведущих племенных заводов, на базе Донского государственного аграрного университета, Поволжского научно-исследовательского института производства и переработки мясомолочной продукции. Для выполнения молекулярно-генетических исследований у овец были взяты биообразцы кожи и хрящевой ткани с ушных раковин путем выщипов площадью 1 см². Оценку проводили методом ПЦР-ПДРФ. Для рестрикции амплифицированных участков генов GDF9, GH были использованы эндонуклеазы BstHH HaeIII. Согласно результатам молекулярно-генетического исследования, определяли наличие и частоту аллелей и генотипов по генам GDF9, GH.

Результаты

Статья посвящена изучению генетической структуры овец волгоградской и эдильбаевской пород. Одним из важных ресурсов создания высокопродуктивных животных является использование маркерной селекции в качестве дополнительного критерия отбора и подбора племенных животных. Использование ДНК-маркеров для отбора животных, содержащих в себе желательные аллели генов хозяйственно-ценных признаков, является одним из новых научных подходов для решения данной проблемы. Развитие овцеводство в нашей стране позволит использовать имеющиеся природные и человеческие ресурсы на селе, а также получать высококачественную баранину путем применения ресурсосберегающих технологий. Целью работы стало изучение генетической структуры овец волгоградской и эдильбаевской пород по генам GDF9, GH. Для выполнения молекулярно-генетических исследований у овец были взяты образцы ушных выщипов (ткани с ушной раковины) площадью 1 см². Оценку проводили методом ПЦР-ПДРФ. Во всех исследованных группах овец установлены А- и В-аллельные варианты гена GDF9, аллели А и В гена GH. Анализ данных выявил породный аспект в распределении частоты аллелей и генотипов генов GDF9, GH в рассматриваемых популяциях.

Заключение

Результаты изучения генетической структуры популяций овец волгоградской и эдильбаевской пород показали, что распределение частот аллелей и генотипов генов гормона роста (GH) и дифференциального фактора роста (GDF9) имеют некоторые особенности, связанные с породной принадлежностью исследуемых овец.

Aim

The study of the genetic structure of sheep of the Volgograd and Edilbaevsky breeds according to the GDF9, GH genes.

Materials and Methods

The research was carried out in herds of sheep from leading breeding plants, on the basis of Don State Agrarian University and Volga Region Research Institute of Manufacture and Processing of Meat-and-Milk Production. Biosamples of skin and cartilage tissue from the auricles were taken from sheep by plucking an area of 1 cm² for molecular genetic studies. Evaluation was performed by PCR-RFLP method. Endonucleases BstHH HaeIII were used to restrict the amplified regions of the GDF9 and GH genes. The presence and frequency of alleles and genotypes for the GDF9, GH genes were determined based on the results of a molecular genetic study.

Results

The article is devoted to the study of the genetic structure of sheep of the Volgograd and Edilbaevsky breeds. One of the important resources for creating highly productive animals is the use of marker selection as an additional criterion for the selection and selection of breeding animals. The use of DNA markers for the selection of animals containing desirable alleles of genes of economically valuable traits is one of the new scientific approaches to solve this problem. The development of sheep breeding in our country will allow using the available natural and human resources in rural areas, as well as obtaining high-quality lamb through the use of resource-saving technologies. The aim of the work was to study the genetic structure of sheep of the Volgograd and Edilbaevsky breeds according to the GDF9, GH genes. To perform molecular genetic studies, samples of ear plucks (tissue from the auricle) with an area of 1 cm2 were taken from sheep. The assessment was carried out by PCR-PDRF. In all the studied groups of sheep, A and B allelic variants of the GDF9 gene, alleles A and B of the GH gene were established. The analysis of the data revealed a breed aspect in the distribution of the frequency of alleles and genotypes of the GDF9, GH genes in the populations under consideration.

Conclusion

The results of the study of the genetic structure of the populations of sheep of the Volgograd and Edilbaevsky breeds showed that the frequency distribution of alleles and genotypes of the growth hormone (GH) and differential growth factor (GDF9) genes have some features associated with the breed affiliation of the studied sheep.

М. И. Сложенкина

Поволжский научно-исследовательский институт производства и переработки мясомолочной продукции, Волгоград, Россия

Марина И. Сложенкина, доктор биологических наук, профессор, член-корреспондент РАН, директор

E-mail: niimmp@mail.ru; тел. 8 (8442) 39-10-48

И. Ф. Горлов

Поволжский научно-исследовательский институт производства и переработки мясомолочной продукции, Волгоград, Россия

Иван Ф. Горлов

Ю. А. Колосов

Донской государственный аграрный университет, пос. Персиановский, Ростовская обл., Россия

Юрий А. Колосов

Н. В. Широкова

Донской государственный аграрный университет, пос. Персиановский, Ростовская обл., Россия

Надежда В. Широкова

About the Authors

M. I. Slozhenkina

Volga Region Research Institute of Manufacture and Processing of Meat-and-Milk Production, Volgograd, Russian Federation

Marina I. Slozhenkina, Dr Biological Sci., Professor, Correspondent member of RAS, Director

E-mail: niimmp@mail.ru; Russia, тел. +7 (8442) 39-10-48

I. F. Gorlov

Volga Region Research Institute of Manufacture and Processing of Meat-and-Milk Production, Volgograd, Russian Federation

Ivan F. Gorlov

Yu. A. Kolosov

Don State Agrarian University, village Persianovsky, Rostov region

Yuriy A. Kolosov

N. V. Shirokova

Don State Agrarian University, village Persianovsky, Rostov region

Nadezhda V. Shirokova

1. Погодаев В.А., Кононова Л.В., Адучиев Б.К. Полиморфизм генов кальпастатина и соматропина у овец калмыцкой курдючной породы и помесей (1/2 калмыцкая курдючная+1/2 дорпер) // Вестник Ульяновской государственной сельскохозяйственной академии. 2019. Том 47. N 3. С. 141-145. DOI: 10.18286/1816-4501-2019-3-141-145

2. Afifi M., Alkaladi A., Kadasa N.M., Saddick S., Baeshen M., Al-Farga A., Aqlan F.M., Almulaiky Y.Q., Alkhaled M., Alayafi A.A.M., Ali H.A., Warsi M.K., Allogmani A.S., Abdelazim A. Sequencing and Polymorphism detection in growth hormone gene in Najdi Sheep and their association with milk production // Journal Of Animal Research. 2019. Vol. 53. P. 423-428. Article Id: B-920. DOI: 10.18805/ijar.B-920

3. Ahani Azari M., Dehnavi E., Yousefi S., Shahmohamadi L.Polymorphism of Calpastatin, Calpain and Myostatin genes in native Dalagh sheep in Iran // Slovak J. Anim. Sci. 2012. Vol. 45. Iss. 1. P. 1-6.

4. Avanus К. Determining genetic variation of Calpastatin gene with MspI and NcoI enzymes by using PCR-RFLP method in Kivircik lambs // Acta Veterinaria Eurasia. 2018. Vol. 44. P. 39-43.

5. Bodensteiner K.J., Clay C.M., Moeller C.L., Sawyer H.R. Molecular cloning of the ovine growth/differentiation factor-9 gene and expression of growth/differentiation factor-9 in ovine and bovine ovaries // Biol Reprod. 1999. Vol. 60. P. 381-386.

6. Georgieva S., Hristova D., Dimitrova I., Stancheva N., Bozhilova-Sakova M. Molecular analysis of ovine calpastatin (CAST) and myostatin (MSTN) genes in Synthetic population Bulgarian Milk sheep using PCR-RFLP // J. BioSci. Biotechnol. 2015. Vol. 4. Iss. 1. P. 95-99.

7. Gorlov I.F., Shirokova N.V., Slozhenkina M.I., Mosolova N.I., Zlobina E.Y., Kolosov Y.A., Getmantseva L.V., Bakoev N.F., Leonova M.A., Kolosov A.Y. Association of the growth hormone gene polymorphism with growth traits in salsk sheep breed // Small Ruminant Research. 2017. Vol. 150. P. 11-14.

8. Gorlov I.F., Kolosov Yu.A., Shirokova N.V., Getmantseva L.V., Slozhenkina M.I., Mosolova N.I., Bakoev N.F., Leonova M.A., Kolosov A.Yu., Zlobina E.Yu. GDF9 gene polymorphism and its association with litter size in two Russian sheep breeds // Rendiconti Lincei. Scienze Fisiche e Naturali. 2018. Vol. 29. Issue 1. Р. 61-66.

9. Gorlov I.F., Filatov A.S., Natyrov A.K., Mosolova N.I., Nikolaev D.V., Nelepov Yu.N., Chamurliev N.G., Vladimtseva I.V., Zlobina E.Yu. Meat productivity of Volgograd breed ram hogs of different genotypes // Research Journal of Pharmaceutikal, Biological and Chemical Sciences. 2018. Vol. 9. Iss. 5. P. 2152-2161.

10. Kolosov Yu.A., Getmantseva L.V., Shirokova N.V., Klimenko A., Bakoev S.Yu., Usatov A.V., Kolosov A.Yu., Bakoev N.F., Leonova M.A. Polymorphism of the GDF9 Gene in Russian Sheep Breeds // J. Cytol. Histol. 2015. Vol. 6. P. 305. DOI: 10.4172/2157-7099.1000305

11. Malewa A.D., Hakim L., Maylinda S., Husain M.H. Growth hormone gene polymorphisms of Indonesia fat tailed sheep using PCR-RFLP and their relationship with growth traits // Livestock Research for Rural Development. 2014. Vol. 26. Iss. 6. URL: http://www.lrrd.org/lrrd26/6/cont2606.htm (дата обращения: 13.04.2021)

12. Moradian C., Mohamadi N., Razavi- Sheshdeh S.A., Hajihosseinlo A., Ashrafi F.Effects of genetic polymorphism the growth hormone gene on growth traits in Makooei sheep // European Journal of Experimental Biology. 2013. Vol. 3. Iss. 3. P. 101-105.

13. Othman L.A., Althwani A.N., Alkhazraji A.J.A.H. Growth hormone gene in Iraqi and Turkish Awassi sheep using PCR-RFLP // World Journal of Pharmaceutical Research. 2016. Vol. 5. Iss. 1. Р. 87-93.

14. Selionova M.I., Plakhtyukova V.R. Polymorphism of the CAPN1 and GH genes and its relationship with the productivity of cattle of the Kazakh white-headed breed // IOP Conference Series: Earth and Environmental Science. 2020. Vol. 613. 012131. DOI:10.1088/1755-1315/613/1/012131

15. Suleman M., Khan S.U., Riaz А.В., Yousaf M., Shah A., Ishaq R., GHafoor A. Calpastatin (CAST) gene polymorphism in Kajli, Lohi and Thalli sheep breeds // Afric. J. of Biotechnol. 2012. Vol. 47. Iss. 11. P. 10655-10660. DOI: 10.5897/ AJB11.2478

16. Szkudlarek-Kowalczyk M., Wiśniewska E., Mroczkowski S. Polymorphisms of Calpastatin gene in sheep // Journal of Central European Agriculture. 2011. Vol. 12. Iss. 3. P. 425-432.

17. Trukhachev V.I., Skripkin V.S., Selionova M.I., Yatsyk O., Krivoruchko A. The polymorphism of REM-1 gene in sheep genome and its influence on some parameters of meat productivity // Research Journal of Pharmaceutical, Biological and Chemical Sciences. 2016. Vol. 7. Iss. 2351. P. 2351-2357.

18. Wang H., Wang J., Wang X., Cheng S., Li X., Wang Z., Fan Q., Fu L., Li S., Zhou X. Association analysis of CAST gene polymorphism with meat quality in five sheep breeds // Acta Agriculturaе Zhejiangensis. 2016. Vol. 28. Iss. 8. P. 1309-1314.

19. Yuldashbayev Yu.A., Shevhuzhev A.F., Kochkarov R.Kh., Mishvelov E.G., Ponomareva A.I. Мeat productivity of young sheep karachai breed // Research Journal of Pharmaceutical, Biological and Chemical Sciences. 2018. Vol. 9. Iss. 4. P. 692-699.

References

1. Pogodaev V.A., Kononova V.L., Aduceau B.K. Polymorphism of calpastatine genes and somatotropin of Kalmyk fat-tailed breed and crossbreed (½ kalmyk зрасте fat-breed + ½ dorper). ULSAU, 2019, vol. 47, no. 3, pp. 141-145. (In Russian) DOI: 10.18286/1816-4501-2019-3-141-145

2. Afifi M., Alkaladi A., Kadasa N.M., Saddick S., Baeshen M., Al-Farga A., Aqlan F.M., Almulaiky Y.Q., Alkhaled M., Alayafi A.A.M., Ali H.A., Warsi M.K., Allogmani A.S., Abdelazim A. Sequencing and Polymorphism detection in growth hormone gene in Najdi Sheep and their association with milk production. Journal of Animal Research, 2019, vol. 53, pp. 423-428. Article Id: B-920. DOI: 10.18805/ijar.B-920

3. Ahani Azari M., Dehnavi E., Yousefi S., Shahmohamadi L.Polymorphism of Calpastatin, Calpain and Myostatin genes in native Dalagh sheep in Iran. Slovak J. Anim. Sci. 2012, vol. 45, iss. 1, pp. 1-6.

4. Avanus К. Determining genetic variation of Calpastatin gene with MspI and NcoI enzymes by using PCR-RFLP method in Kivircik lambs. Acta Veterinaria Eurasia. 2018, vol. 44, pp. 39-43.

5. Bodensteiner K.J., Clay C.M., Moeller C.L., Sawyer H.R. Molecular cloning of the ovine growth/differentiation factor-9 gene and expression of growth/differentiation factor-9 in ovine and bovine ovaries. Biol Reprod. 1999, vol. 60, pp. 381-386.

6. Georgieva S., Hristova D., Dimitrova I., Stancheva N., Bozhilova-Sakova M. Molecular analysis of ovine calpastatin (CAST) and myostatin (MSTN) genes in Synthetic population Bulgarian Milk sheep using PCR-RFLP. J. BioSci. Biotechnol. 2015, vol. 4, iss. 1, pp. 95-99.

7. Gorlov I.F., Shirokova N.V., Slozhenkina M.I., Mosolova N.I., Zlobina E.Y., Kolosov Y.A., Getmantseva L.V., Bakoev N.F., Leonova M.A., Kolosov A.Y. Association of the growth hormone gene polymorphism with growth traits in salsk sheep breed. Small Ruminant Research. 2017, vol. 150, pp. 11-14.

8. Gorlov I.F., Kolosov Yu.A., Shirokova N.V., Getmantseva L.V., Slozhenkina M.I., Mosolova N.I., Bakoev N.F., Leonova M.A., Kolosov A.Yu., Zlobina E.Yu. GDF9 gene polymorphism and its association with litter size in two Russian sheep breeds. Rendiconti Lincei. Scienze Fisiche e Naturali. 2018, vol. 29, issue 1, pp. 61-66.

9. Gorlov I.F., Filatov A.S., Natyrov A.K., Mosolova N.I., Nikolaev D.V., Nelepov Yu.N., Chamurliev N.G., Vladimtseva I.V., Zlobina E.Yu. Meat productivity of Volgograd breed ram hogs of different genotypes. Research Journal of Pharmaceutikal, Biological and Chemical Sciences. 2018, vol. 9, iss. 5, pp. 2152-2161.

10. Kolosov Yu.A., Getmantseva L.V., Shirockova N.V., Klimenko A., Bakoev S.Yu., Usatov A.V., Kolosov A.Yu., Bakoev N.F., Leonova M.A. Polymorphism of the GDF9 Gene in Russian Sheep Breeds. J. Cytol. Histol, 2015, vol. 6, pp. 305. DOI: 10.4172/2157-7099.1000305

11. Malewa A.D., Hakim L., Maylinda S., Husain M.H. Growth hormone gene polymorphisms of Indonesia fat tailed sheep using PCR-RFLP and their relationship with growth traits. Livestock Research for Rural Development. 2014, vol. 26, iss. 6. Available at: http://www.lrrd.org/lrrd26/6/cont2606.htm (accessed 13.04.2021)

12. Moradian C., Mohamadi N., Razavi- Sheshdeh S.A., Hajihosseinlo A., Ashrafi F.Effects of genetic polymorphism the growth hormone gene on growth traits in Makooei sheep // European Journal of Experimental Biology. 2013. Vol. 3. Iss. 3. P. 101-105.

13. Othman L.A., Althwani A.N., Alkhazraji A.J.A.H. Growth hormone gene in Iraqi and Turkish Awassi sheep using PCR-RFLP // World Journal of Pharmaceutical Research. 2016. Vol. 5. Iss. 1. Р. 87-93.

14. Selionova M.I., Plakhtyukova V.R. Polymorphism of the CAPN1 and GH genes and its relationship with the productivity of cattle of the Kazakh white-headed breed // IOP Conference Series: Earth and Environmental Science. 2020. Vol. 613. 012131. DOI:10.1088/1755-1315/613/1/012131

15. Suleman M., Khan S.U., Riaz А.В., Yousaf M., Shah A., Ishaq R., GHafoor A. Calpastatin (CAST) gene polymorphism in Kajli, Lohi and Thalli sheep breeds // Afric. J. of Biotechnol. 2012. Vol. 47. Iss. 11. P. 10655-10660. DOI: 10.5897/ AJB11.2478

16. Szkudlarek-Kowalczyk M., Wiśniewska E., Mroczkowski S. Polymorphisms of Calpastatin gene in sheep // Journal of Central European Agriculture. 2011. Vol. 12. Iss. 3. P. 425-432.

17. Trukhachev V.I., Skripkin V.S., Selionova M.I., Yatsyk O., Krivoruchko A. The polymorphism of REM-1 gene in sheep genome and its influence on some parameters of meat productivity // Research Journal of Pharmaceutical, Biological and Chemical Sciences. 2016. Vol. 7. Iss. 2351. P. 2351-2357.

18. Wang H., Wang J., Wang X., Cheng S., Li X., Wang Z., Fan Q., Fu L., Li S., Zhou X. Association analysis of CAST gene polymorphism with meat quality in five sheep breeds // Acta Agriculturaе Zhejiangensis. 2016. Vol. 28. Iss. 8. P. 1309-1314.

19. Yuldashbayev Yu.A., Shevhuzhev A.F., Kochkarov R.Kh., Mishvelov E.G., Ponomareva A.I. Мeat productivity of young sheep karachai breed // Research Journal of Pharmaceutical, Biological and Chemical Sciences. 2018. Vol. 9. Iss. 4. P. 692-699.