 Тихонов С.Л.,Тихонова Н.В.,Лазарев В.А., Тихонова М.С.
https://doi.org/10.31208/2618-7353-2022-19-60-68
АННОТАЦИЯ / ABSTRACT
Цель
Оценка антиоксидантной активности пептидов, выделенных из трипсинового гидролизата молозива коров.
Материалы и методы
Антиоксидантную активность пептидов определяли по методу DPPH. В качестве стандартного раствора использовали растворы Тролокса (6-гидрокси-2,5,7,8-тетраметилхроман-2-карбоновой кислоты) известной концентрации. Все спектрофотометрические измерения проводили с использованием микропланшетного ридера CLARIOstar (BMG Labtech, Германия).
Результаты
Проведены исследования антиоксидантной активности пептидов с известной молекулярной массой и последовательностью аминокислот, выделенных из ферментативного гидролизата молозива коров. В научно-технической отечественной и зарубежной литературе функции указанных пептидов не представлены. Антиоксидантную активность определяли по методу DPPH. Установлено, что пептид с аминокислотной последовательностью SQKKKNCPNGTRIRVPGPGP, состоящий из 20 аминокислот и имеющий молекулярную массу 20 кДа, обладает антиоксидантной активностью 0,128±0,008 ммоль экв. Тролокса/л, у других исследованных пептидов антиоксидантные свойства не установлены. Можно предположить, что антиоксидантная активность пептидов зависит от последовательности и количества аминокислот.
Заключение
На основании полученных данных можно рассмотреть возможность использования указанного пептида при разработке пищевых продуктов функциональной направленности. Однако следует учитывать, что биологически активные пептиды имеют ряд недостатков, в частности, низкую стабильность в желудочно-кишечном тракте, и могут вступать в реакции с другими биологически активными веществами в составе пищевого продукта и в организме человека.
Purpose
Evaluation of the antioxidant activity of peptides isolated from trypsin hydrolysate of cow colostrum.
Materials and Methods
Based on the data obtained, it is possible to consider the possibility of using this peptide in the development of functional food products. However, it should be borne in mind that biologically active peptides have a number of disadvantages, in particular, low stability in the gastrointestinal tract, and can react with other biologically active substances in the composition of a food product and in the human body.
Results
The antioxidant activity of peptides was determined by the DPPH method. Solutions of Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) of known concentration were used as the standard solution. All spectrophotometric measurements were carried out using a CLARIOstar micro-tablet reader (BMG Labtech, Germany).Studies of the antioxidant activity of peptides with a known molecular weight and sequence of amino acids isolated from enzymatic hydrolysate of cow colostrum have been carried out. The functions of these peptides are not presented in the scientific and technical domestic and foreign literature. The antioxidant activity was determined by the DPPH method. It was found that the peptide with the amino acid sequence SQKKKNCPNGTRIRVPGPGP, consisting of 20 amino acids and having a molecular weight of 20 kDa, has an antioxidant activity of 0.128±0.008 mmol eq. Trolox/l, the antioxidant properties of the other peptides studied have not been established. It can be assumed that the antioxidant activity of peptides depends on the sequence and number of amino acids.
Conclusion
Based on the data obtained, it is possible to consider the possibility of using this peptide in the development of functional food products. However, it should be borne in mind that biologically active peptides have a number of disadvantages, in particular, low stability in the gastrointestinal tract, and can react with other biologically active substances in the composition of a food product and in the human body.
АВТОРЫ / AUTHORS
С. Л. Тихонов
Уральский государственный экономический университет, 620144, Россия, Екатеринбург, ул. 8 Марта/Народной Воли, д. 62/45
Тихонов Сергей Леонидович, доктор технических наук, профессор, заведующий кафедрой пищевой инженерии
E-mail: tihonov75@bk.ru; тел.: 8 (343) 283-11-38
Н. В. Тихонова
Уральский государственный экономический университет 620144, Россия, Екатеринбург, ул. 8 Марта/Народной Воли, д. 62/45
Тихонова Наталья Валерьевна, доктор технических наук, профессор, профессор кафедры пищевой инженерии
Е-mail: tihonov75@bk.ru
В. А. Лазарев
Уральский государственный экономический университет, 620144, Россия, Екатеринбург, ул. 8 Марта/Народной Воли, д. 62/45
Лазарев Владимир Александрович, кандидат технических наук, доцент кафедры пищевой инженерии
Е-mail: lazarev.eka@gmail.com
М. С. Тихонова
Уральский государственный медицинский университет, 620028, г. Екатеринбург, ул. Репина, д. 3
Тихонова Мария Сергеевна, студентка
Е-mail: lazarev.eka@gmail.com
About the Authors
S. L. Tikhonov
Ural State University of Economics 45, People's Will st., Ekaterinburg, 620144, Russian Federation
Sergey L. Tikhonov, Dr. Sci. (Technology), Professor, Head of the Department of Food Engineering
E-mail: tihonov75@bk.ru; tel.: +7 (343) 283-11-38
N. V. Tikhonova
Ural State University of Economics 45, People's Will st., Ekaterinburg, 620144, Russian Federation
Natalia V. Tikhonova, Dr. Sci. (Technology), Professor of the Department of Food Engineering
Е-mail: tihonov75@bk.ru
V. A. Lazarev
Ural State University of Economics 45, People's Will st., Ekaterinburg, 620144, Russian Federation
Vladimir A. Lazarev, PhD (Technology), Associate Professor of the Department of Food Engineering
Е-mail: lazarev.eka@gmail.com
M. S. Tikhonova
Ural State Medical University 3, Repin st., Ekaterinburg, 620028, Russian Federation
Maria S. Tikhonova, Student
Е-mail: lazarev.eka@gmail.com
СПИСОК ИСТОЧНИКОВ / REFERENCES
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8. Parodi PW. A role for milk proteins and their peptides in cancer prevention // Current Pharmaceutical Design. 2007. Vol. 13, 8. P. 813-828; https://doi.org/10.2174/138161207780363059.
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References
1. Díaz-Gómez JL, Neundorf I, López-Castillo LM, Castorena-Torres F, Serna-Saldívar SO, García-Lara S. In Silico Analysis and In Vitro Characterization of the Bioactive Profile of Three Novel Peptides Identified from 19 kDa α-Zein Sequences of Maize // Molecules. 2020. Vol. 25, iss. 22. Article number: 5405. https://doi.org/10.3390/molecules25225405.
2. Iwaniak A, Mogut D, Minkiewicz P, Żulewska J, Darewicz M. An integrated approach to the analysis of antioxidative peptides derived from Gouda cheese with a modified β-casein content // Sci Rep. 2022. Aug 3. Vol. 12, iss. 1. Article number: 13314. https://doi.org/10.1038/s41598-022-17641-x.
3. Krobthong S, Yingchutrakul Y, Sittisaree W et al. Evaluation of potential anti-metastatic and antioxidative abilities of natural peptides derived from Tecoma stans (L.) Juss. ex Kunth in A549 cells // PeerJ. 2022. Jul 6. Vol. 10. Article number: e13693. https://doi.org/10.7717/peerj.13693.
4. Lemes AC, Sala L, Ores J, Braga ARC, Egea M, Fernandes KF. A Review of the Latest Advances in Encrypted Bioactive Peptides from Protein-Rich Waste // International Journal of Molecular Sciences. 2016. Vol. 17, iss. 6. Article number: 950. https://doi.org/10.3390/ijms17060950.
5. Lopez-Exposito I, Recio I. Protective effect of milk peptides: antibacterial and antitumor properties // Advances in Experimental Medicine and Biology. 2008. Vol. 606. P. 271-293. https://doi.org/10.1007/978-0-387-74087-4_11.
6. Manzanares P, Gandía M, Garrigues S, Marcos JF. Improving Health-Promoting Effects of Food-Derived Bioactive Peptides through Rational Design and Oral Delivery Strategies // Nutrients. 2019. Vol. 11, 10. Article number:2545. https://doi.org/10.3390/nu11102545.
7. Menchetti L, Traina G, Tomasello G et al. Potential benefits of colostrum in gastrointestinal diseases // Frontiers in Bioscience-Scholar. 2016. Vol. 8, 2. P. 331-351. https://doi.org/10.2741/s467.
8. Parodi PW. A role for milk proteins and their peptides in cancer prevention // Current Pharmaceutical Design. 2007. Vol. 13, 8. P. 813-828; https://doi.org/10.2174/138161207780363059.
9. Pérez-Gregorio R, Soares S, Mateus N, de Freitas V. Bioactive Peptides and Dietary Polyphenols: Two Sides of the Same Coin // Molecules. 2020. Vol. 25, 15. Article number: 3443. https://doi.org/10.3390/molecules25153443.
10. Pyo YH, Jin YJ, Hwang JY. Comparison of the effects of blending and juicing on the phytochemicals contents and antioxidant capacity of typical Korean kernel fruit juices // Preventive nutrition and food science. 2014. Vol. 19, 2. P. 108-114; https://doi.org/10.3746/pnf.2014.19.2.108.
11. Ucak I, Afreen M, Montesano D et al. Functional and Bioactive Properties of Peptides Derived from Marine Side Streams // Mar Drugs. 2021. Vol. 19, 2. Article number: 71. https://doi.org/10.3390/md19020071.
12. Wu R, Wu C, Liu D, Yang X, Huang J, Zhang J, Liao B, He H, Li H. Overview of Antioxidant Peptides Derived from Marine Resources: The Sources, Characteristic, Purification, and Evaluation Methods // Applied biochemistry and biotechnology. 2015. Vol. 176, 7. P. 1815-1833. https://doi.org/10.1007/s12010-015-1689-9.
Ключевые слова:
пептиды, молозиво коров, антиоксидантная активность, ферментативный гидролиз, аминокислоты, молекулярная масса
Key words:
peptides, cow colostrum, antioxidant activity, enzymatic hydrolysis, amino acids, molecular weight
Для цитирования:
Тихонов С.Л., Тихонова Н.В., Лазарев В.А., Тихонова М.С. Исследование антиоксидантных свойств пептидов, выделенных из молозива коров // Аграрно-пищевые инновации. 2022. Т. 19, № 3. С. 60-68. https://doi.org/10.31208/2618-7353-2022-19-60-68.
For citation:
Tikhonov S.L., Tikhonova N.V., Lazarev V.A., Tikhonova M.S. Study of antioxidant properties of peptides, isolated from cows colostrum. Agrarian-and-food innovations. 2022;19(3):60-68. (In Russ.). https://doi.org/10.31208/2618-7353-2022-19-60-68. | 